Regulation of exocytotic fusion by cell inflation.

  title={Regulation of exocytotic fusion by cell inflation.},
  author={Carles Solsona and Barbara Innocenti and J. M. Fern{\'a}ndez},
  journal={Biophysical journal},
  volume={74 2 Pt 1},
We have inflated patch-clamped mast cells by 3.8 +/- 1.6 times their volume by applying a hydrostatic pressure of 5-15 cm H2O to the interior of the patch pipette. Inflation did not cause changes in the cell membrane conductance and caused only a small reversible change in the cell membrane capacitance (36 +/- 5 fF/cm H2O). The specific cell membrane capacitance of inflated cells was found to be 0.5 microF/cm2. High-resolution capacitance recordings showed that inflation reduced the frequency… CONTINUE READING