Regulation of enzyme activity in the cell: effect of enzyme concentration.

@article{Aragon1991RegulationOE,
  title={Regulation of enzyme activity in the cell: effect of enzyme concentration.},
  author={Juan Aragon and Alberto Sols},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  year={1991},
  volume={5 14},
  pages={
          2945-50
        }
}
The rapid development in our understanding of the regulation of enzyme activity makes it a high priority to ascertain whether the behavior of purified enzymes reflects their functional characteristics in vivo. Enzyme concentration is usually the most significant difference between routine in vitro assays and in vivo conditions, as it is well known that many intracellular enzymes are present in vivo at much higher concentrations than used in vitro. Various procedures are suitable for kinetic… CONTINUE READING
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