Regulation of directionality in bacteriophage lambda site-specific recombination: structure of the Xis protein.

@article{Sam2002RegulationOD,
  title={Regulation of directionality in bacteriophage lambda site-specific recombination: structure of the Xis protein.},
  author={My D. Sam and Christie V Papagiannis and Kevin M. Connolly and Leah Corselli and Junji Iwahara and James Chun-Yin Lee and Martin Phillips and Jonathan M. Wojciak and Reid C. Johnson and Robert T Clubb},
  journal={Journal of molecular biology},
  year={2002},
  volume={324 4},
  pages={791-805}
}
Upon induction of a bacteriophage lambda lysogen, a site-specific recombination reaction excises the phage genome from the chromosome of its bacterial host. A critical regulator of this process is the phage-encoded excisionase (Xis) protein, which functions both as a DNA architectural factor and by cooperatively recruiting integrase to an adjacent binding site specifically required for excision. Here we present the three-dimensional structure of Xis and the results of a structure-based… CONTINUE READING