Regulation of cytochrome P450 expression in Drosophila: Genomic insights.

@article{Giraudo2010RegulationOC,
  title={Regulation of cytochrome P450 expression in Drosophila: Genomic insights.},
  author={Maeva Giraudo and Gopalan C. Unnithan and Ga{\"e}lle Le Goff and Ren{\'e} Feyereisen},
  journal={Pesticide biochemistry and physiology},
  year={2010},
  volume={97 2},
  pages={
          115-122
        }
}
View on PubMed
europepmc.org
67 Citations
Highly Influential Citations
1
Background Citations
26
Methods Citations
1
Results Citations
2

Figures and Tables from this paper

67 Citations

Induction of a detoxification gene in Drosophila melanogaster requires an interaction between tissue specific enhancers and a novel cis-regulatory element.
Boric Acid Resistance in Drosophila melanogaster Depends on the Expression Level of the Cyp9b2 Gene
TLDR
This work investigated the role of a cluster of two highly homologous genes Cyp9B1 and Cyp9b2 in the resistance of D. melanogaster to boric acid, which correlates with in silico analysis demonstrating the different regulatory potential of 5'-regulatory regions of these genes.
Use of Mutagenesis, Genetic Mapping and Next Generation Transcriptomics to Investigate Insecticide Resistance Mechanisms
TLDR
A genome-wide insertional mutagenesis screen to Drosophila melanogaster, using a Minos-based construct, and retrieved a line resistant to the neonicotinoid insecticide Imidacloprid confirmed that resistance was due to increased P450 detoxification.
Identification of SNPs involved in regulating a novel alternative transcript of P450 CYP6ER1 in the brown planthopper
TLDR
A novel alternative transcript of CYP6ER1 (transcript A2) was identified that had different expression patterns between resistant and susceptible populations, and was more stable after insecticide induction, providing novel evidence regarding the transcriptional regulation of a metabolic resistance‐related gene.
Genomic and transcriptomic analyses in Drosophila suggest that the ecdysteroid kinase-like (EcKL) gene family encodes the 'detoxification-by-phosphorylation' enzymes of insects.
Cytochrome P450s Cyp4p1 and Cyp4p2 associated with the DDT tolerance in the Drosophila melanogaster strain 91-R.
Comparative CYP-omic analysis between the DDT-susceptible and -resistant Drosophila melanogaster strains 91-C and 91-R.
TLDR
The results demonstrate that a combination of changes in P450 protein-coding regions and transcript levels are possibly associated with DDT resistance, and suggest that selection for variant function may occur within this gene family in response to chronic DDT exposure.
THE COMPLEX GENETICS OF LIFESPAN AND XENOBIOTIC RESISTANCE IN DROSOPHILA MELANOGASTER
TLDR
The studies demonstrate the genetic architecture of lifespan is more complex than what is reported and lose of function variants may have an important role in creating variable effects on xenobiotic resistance.
Structure and Function of Cytochrome P450S in Insect Adaptation to Natural and Synthetic Toxins: Insights Gained from Molecular Modeling
TLDR
Differences in multiple regions of the insect proteins reveal the evolutionary processes occurring as P450 genes have duplicated and diverged, and can yield insights into plant-insect coevolution and into novel approaches for chemical pest management.
The effect of insecticide synergist treatment on genome-wide gene expression in a polyphagous pest
Synergists can counteract metabolic insecticide resistance by inhibiting detoxification enzymes or transporters. They are used in commercial formulations of insecticides, but are also frequently used…
...
...

References

SHOWING 1-10 OF 66 REFERENCES
Inducibility of the Drosophila melanogaster cytochrome P450 gene, CYP6A2, by phenobarbital in insecticide susceptible or resistant strains.
Xenobiotic response in Drosophila melanogaster: sex dependence of P450 and GST gene induction.
Metabolism of promutagens catalyzed by Drosophila melanogaster CYP6A2 enzyme in Saccharomyces cerevisiae
TLDR
The somatic mutation and recombination test (SMART) in Drosophila melanogaster allows screening of chemicals for genotoxicity in a multicellular organism and CYP6A2 mRNA was found to be present in the insecticide‐resistant strain at higher levels than in the Insecticide‐sensitive strain, suggesting insecticide resistance might be correlated with enhanced CYP 6A2 expression.
Induction of two cytochrome P450 genes, Cyp6a2 and Cyp6a8, of Drosophila melanogaster by caffeine in adult flies and in cell culture.
A comparison of Drosophila melanogaster detoxification gene induction responses for six insecticides, caffeine and phenobarbital.
Characterization of Drosophila melanogaster cytochrome P450 genes
TLDR
This work characterized Drosophila melanogaster P450 expression patterns in embryos and 2 stages of third instar larvae and identified numerous P450s expressed in the fat body, Malpighian (renal) tubules, and in distinct regions of the midgut, consistent with hypothesized roles in detoxification processes.
The Drosophila cytochrome P450 gene Cyp6a2: structure, localization, heterologous expression, and induction by phenobarbital.
TLDR
Results show that CYP6A2 catalyzes the metabolism of organophosphorus insecticides and they implicate Cyp6a2 overexpression in metabolic resistance.
A Drosophila systems approach to xenobiotic metabolism.
TLDR
It is shown that, although detoxification enzymes are widely distributed, baseline protection against DDT resides primarily in the insect excretory system, corresponding to less than 0.1% of the mass of the organism.
The DHR96 nuclear receptor regulates xenobiotic responses in Drosophila.
Constitutive and barbital-induced expression of the Cyp6a2 allele of a high producer strain of CYP6A2 in the genetic background of a low producer strain.
...
...