Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto‐induced peptide pheromone and a two‐component regulatory system

  title={Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto‐induced peptide pheromone and a two‐component regulatory system},
  author={Ekaterina V. Pestova and Leiv Sigve H{\aa}varstein and Donald A. Morrison},
  journal={Molecular Microbiology},
The regulation of competence for genetic transformation in Streptococcus pneumoniae depends on a quorum‐sensing system, but the only molecular elements of the system whose specific role have been identified are an extracellular peptide signal and an ABC‐transporter required for its export. Here we show that transcription of comC, the gene encoding a predicted 41‐residue precursor peptide that is thought to be processed and secreted as the 17‐residue mature competence activator, increased… 
Cooperative Regulation of Competence Development in Streptococcus pneumoniae: Cell-to-Cell Signaling via a Peptide Pheromone and an Alternative Sigma Factor
This chapter outlines the up-regulation of genes in response to the competence-stimulating peptide (CSP) , as well as a number of recent discoveries indicating that the coordination of gene
Inhibition of competence development in Streptococcus pneumoniae by increased basal-level expression of the ComDE two-component regulatory system.
Evidence is presented that increased basal-level expression of comDE impedes both spontaneous and CSP-induced competence in S. pneumoniae.
Constitutive competence for genetic transformation in Streptococcus pneumoniae caused by mutation of a transmembrane histidine kinase
Competence for DNA uptake and genetic transformation in Streptococcus pneumoniae is regulated by a quorum‐sensing system. A competence‐stimulating polypeptide (CSP) is secreted by the bacteria and
The com locus controls genetic transformation in Streptococcus pneumoniae
In mixed‐culture experiments consisting of isogenic pairs of pneumococci (Csp+ and Csp−), it was showed that induction of competence by quorum sensing was independent of CSP and that ComD and ComE are required for this CSP‐dependent expression.
Identification of ComW as a new component in the regulation of genetic transformation in Streptococcus pneumoniae
ComX protein accumulation and expression of a late competence gene in the above strain support the conclusion that ComW is a new positive factor involved in competence regulation.
Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays
It is established that among 124 CSP‐inducible genes, 67 were individually dispensable for transformation, whereas 23 were required for transformation.
Inhibition of Competence Development, Horizontal Gene Transfer and Virulence in Streptococcus pneumoniae by a Modified Competence Stimulating Peptide
These results demonstrate the applicability of generating competitive analogues of CSPs as drugs to control horizontal transfer of antibiotic resistance and virulence genes, and to attenuate virulence during infection by S. pneumoniae.
Cross‐regulation of competence pheromone production and export in the early control of transformation in Streptococcus pneumoniae
Data indicate that pheromone autoinduction, cross‐regulation of the comAB and comCDE operons and, possibly, competence shut‐off contribute to the early control of competence development in S. pneumoniae.
Identification of DNA binding sites for ComE, a key regulator of natural competence in Streptococcus pneumoniae
It is shown that ComE is a DNA‐binding protein that acts autocatalytically by binding to a region in its own promoter by activating transcription of the late genes indirectly through one or more intermediate factors.
Identification of a New Regulator inStreptococcus pneumoniae Linking Quorum Sensing to Competence for Genetic Transformation
It is concluded that the factor is a competence-specific global transcription modulator which links quorum-sensing information transduced to ComE to competence and proposed that it acts as an alternate sigma factor.


Convergent sensing pathways mediate response to two extracellular competence factors in Bacillus subtilis.
Characterization of a second extracellular competence stimulating factor (CSF) appears to be, at least in part, a small peptide of between 520 and 720 daltons, which might function to integrate different physiological signals.
Competence pheromone, oligopeptide permease, and induction of competence in Streptococcus pneumoniae
The hypothesis that CSP signal transmission rather involves a two‐component regulatory system is favoured and a general hypothesis is proposed to account for the modulation of competence by peptide permease mutants in S. pneumoniae.
An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae.
It is shown that strain CP1200 produces a 17-residue peptide that induces cells of the Streptococcus pneumoniae species to develop competence and the hypothesis is presented that this transport protein is encoded by comA, previously shown to be required for elaboration of the pneumococcal competence activator.
Competence for genetic transformation in pneumococcus depends on synthesis of a small set of proteins
Pulse-labelling studies show that for the brief period of competence protein synthesis is restricted to a few specific polypeptides, and the response to competence factor has been shown to require protein synthesis.
Cell density control of staphylococcal virulence mediated by an octapeptide pheromone.
  • G. Ji, R. Beavis, R. Novick
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1995
This study has demonstrated that the synthesis of Staphylococcus aureus virulence factors is controlled by a density-sensing system that utilizes an octapeptide produced by the organism itself.
The genes involved in production of and immunity to sakacin A, a bacteriocin from Lactobacillus sake Lb706
Northern (RNA) blot analysis revealed that the putative SapK/SapR system probably acts as a transcriptional activator on both operons, and a 35-bp sequence was shown to be necessary for proper expression and could be possible targets for transcriptional activation.
Protein phosphorylation and regulation of adaptive responses in bacteria.
An attempt is made to understand how cross-talk between parallel phosphotransfer pathways can provide a global regulatory curcuitry.
Identification and molecular analysis of a locus that regulates extracellular toxin production in Clostridium perfringens
Virulence studies carried out using a mouse model implicated the virS gene in the pathogenesis of histotoxic C. perfringens infections and concluded that a two‐component sensor regulator system that activated the expression of a number of extracellular toxins and enzymes involved In virulence had been cloned and sequenced.