Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto‐induced peptide pheromone and a two‐component regulatory system

@article{Pestova1996RegulationOC,
  title={Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto‐induced peptide pheromone and a two‐component regulatory system},
  author={Ekaterina V. Pestova and Leiv Sigve H{\aa}varstein and Donald A. Morrison},
  journal={Molecular Microbiology},
  year={1996},
  volume={21}
}
The regulation of competence for genetic transformation in Streptococcus pneumoniae depends on a quorum‐sensing system, but the only molecular elements of the system whose specific role have been identified are an extracellular peptide signal and an ABC‐transporter required for its export. Here we show that transcription of comC, the gene encoding a predicted 41‐residue precursor peptide that is thought to be processed and secreted as the 17‐residue mature competence activator, increased… 
Cooperative Regulation of Competence Development in Streptococcus pneumoniae: Cell-to-Cell Signaling via a Peptide Pheromone and an Alternative Sigma Factor
This chapter outlines the up-regulation of genes in response to the competence-stimulating peptide (CSP) , as well as a number of recent discoveries indicating that the coordination of gene
Inhibition of competence development in Streptococcus pneumoniae by increased basal-level expression of the ComDE two-component regulatory system.
TLDR
Evidence is presented that increased basal-level expression of comDE impedes both spontaneous and CSP-induced competence in S. pneumoniae.
Constitutive competence for genetic transformation in Streptococcus pneumoniae caused by mutation of a transmembrane histidine kinase
Competence for DNA uptake and genetic transformation in Streptococcus pneumoniae is regulated by a quorum‐sensing system. A competence‐stimulating polypeptide (CSP) is secreted by the bacteria and
The com locus controls genetic transformation in Streptococcus pneumoniae
TLDR
In mixed‐culture experiments consisting of isogenic pairs of pneumococci (Csp+ and Csp−), it was showed that induction of competence by quorum sensing was independent of CSP and that ComD and ComE are required for this CSP‐dependent expression.
Identification of ComW as a new component in the regulation of genetic transformation in Streptococcus pneumoniae
TLDR
ComX protein accumulation and expression of a late competence gene in the above strain support the conclusion that ComW is a new positive factor involved in competence regulation.
Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays
TLDR
It is established that among 124 CSP‐inducible genes, 67 were individually dispensable for transformation, whereas 23 were required for transformation.
Inhibition of Competence Development, Horizontal Gene Transfer and Virulence in Streptococcus pneumoniae by a Modified Competence Stimulating Peptide
TLDR
These results demonstrate the applicability of generating competitive analogues of CSPs as drugs to control horizontal transfer of antibiotic resistance and virulence genes, and to attenuate virulence during infection by S. pneumoniae.
Cross‐regulation of competence pheromone production and export in the early control of transformation in Streptococcus pneumoniae
TLDR
Data indicate that pheromone autoinduction, cross‐regulation of the comAB and comCDE operons and, possibly, competence shut‐off contribute to the early control of competence development in S. pneumoniae.
Identification of DNA binding sites for ComE, a key regulator of natural competence in Streptococcus pneumoniae
TLDR
It is shown that ComE is a DNA‐binding protein that acts autocatalytically by binding to a region in its own promoter by activating transcription of the late genes indirectly through one or more intermediate factors.
Identification of a New Regulator inStreptococcus pneumoniae Linking Quorum Sensing to Competence for Genetic Transformation
TLDR
It is concluded that the factor is a competence-specific global transcription modulator which links quorum-sensing information transduced to ComE to competence and proposed that it acts as an alternate sigma factor.
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