Regulation of colominic acid biosynthesis by temperature: Role of cytidine 5′‐monophosphate N‐acetylneuraminic acid synthetase

  title={Regulation of colominic acid biosynthesis by temperature: Role of cytidine 5′‐monophosphate N‐acetylneuraminic acid synthetase},
  author={C. Gonz{\'a}lez-Clemente and J. Luengo and L. Rodr{\'i}guez-Aparicio and {\'A}. Reglero},
  journal={FEBS Letters},
Synthesis of colominic acid in Escherichia coli K‐235 is strictly regulated by temperature. Evidence for the role of cytidine 5′‐monophospho‐N‐acetylneuraminic acid (CMP‐Neu5Ac) synthetase in this regulation was obtained by measuring its level in E. coli grown at 20 and 37°C. No activity was found in E. coli grown at 20°C. CMP‐Neu5Ac started to be quickly synthesized when bacteria grown at 20°C were transferred to 37°C and was halted when cells grown at 37°C were transferred to 20°C. These… Expand
Regulation of capsular polysialic acid biosynthesis by N‐acetyl‐d‐mannosamine, an intermediate of sialic acid metabolism
Results indicate that ManNAc‐6‐phosphate is able to regulate NeuAc lyase activity and modulate the PA synthesis. Expand
Regulation of capsular polysialic acid biosynthesis by temperature in Pasteurella haemolytica A2
Findings indicate that the cellular growth temperature regulates the synthesis of these enzymes and hence the concentration of the intermediates necessary for capsular polysaccharide genesis in P. haemolytica A2. Expand
Uptake of N‐acetyl‐D‐mannosamine: an essential intermediate in polysialic acid biosynthesis by Escherichia coli K92
Study of specificities showed that N‐acetylglucosamine and mannosamine specifically inhibited the transport of ManNAc in this bacterium, which strongly support the notion that Man NAc uptake depends on a specific phosphotransferase system. Expand
Temperature has reciprocal effects on colanic acid and polysialic acid biosynthesis in E. coli K92
The study of physicochemical conditions required for CA synthesis revealed that in E. coli K92, nutrient modulates CA production, reaching the maximal values when glucose and proline were as carbon and nitrogen sources, respectively. Expand
Transport of N-acetyl-D-galactosamine in Escherichia coli K92: effect on acetyl-amino sugar metabolism and polysialic acid production.
Results strongly support the notion that GalNAc uptake depends on a specific phosphotransferase system and show that E. coli K92 has developed a regulation mechanism that specifically induces the appropriate permease based on the presence of each respective phospho-amino sugar. Expand
Transport of N‐acetyl‐D‐mannosamine and N‐acetyl‐D‐glucosamine in Escherichia coli K1: effect on capsular polysialic acid production
The ManNAc and the GlcNAc phosphorylation that occurs during uptake affected NeuAc synthesis in vitro and account for the low in vivo PA production observed when E. coli K1 uses Man NAc or Glc NAc as a carbon source for growth. Expand
Biosynthesis and production of polysialic acids in bacteria
Polysialic acids (PA) are protective capsular sialohomopolymers present in some bacteria which can invade the mammalian host and cause lethal bacteremia and meningitis. Biosynthesis and translocationExpand
Purification and characterization of GlcNAc-6-P 2-epimerase from Escherichia coli K92.
The results indicate that, unlike UDP-GlcNAc 2-epimerase, which promotes the biosynthesis of sialic acid, Glc NAc-6-P 2-EPimerase plays a catabolic role. Expand
Author index to volume 250
  • 2001
Abad, C. see MC. Bat% Abe, M. see S. Tsunekawa Acan, N.L. and E.F. Tezcan, Sheep brain glu, tathione reductase: purification and general properties Aceto, A. see C. Di Ilio Adelmann-Grill, B. see C.Expand


Regulation of sialic acid metabolism in Escherichia coli: role of N-acylneuraminate pyruvate-lyase
It is shown that sialic acid aldolase can regulate the intracellular concentration of sIALic acid and therefore the concentration of CMP-NeuNAc, and direct evidence for the central role of a Aldolase in regulating the metabolic flux of siali adid in E. coli is provided. Expand
The sialic acids. X. Purification and properties of cytidine 5'-monophosphosialic acid synthetase.
The chemical synthesis of N-acetylneuraminic acid hydroxamate is described, and reversibility of the reaction was detected, i.e. the enzyme catalyzed the incorporation of 32PPi into CTP in complete incubation mixtures. Expand
In vitro synthesis of colominic acid by membrane-bound sialyltransferase of Escherichia coli K-235. Kinetic properties of this enzyme and inhibition by CMP and other cytidine nucleotides.
The membrane-bound sialyltransferase obtained from Escherichia coli K-235 grown in a chemically defined medium (ideal for colominic acid production) was studied and CMP inhibition corresponded to a competitive model and the calculated Ki was 30 microM. Expand
Purification, properties, and genetic location of Escherichia coli cytidine 5'-monophosphate N-acetylneuraminic acid synthetase.
The purified CMP-NeuAc synthetase appears to be identical to the 50,000 Mr polypeptide encoded by this gene based on insertion mutations that result in the loss of detectable enzymatic activity. Expand
Role of a membranous sialyltransferase complex in the synthesis of surface polymers containing polysialic acid in Escherichia coli. Temperature-induced alteration in the assembly process.
Using reconstruction experiments, the results establish that the temperature-induced alteration in polymer synthesis resulted from the inability of cells grown at 15 degrees C to either synthesize or assemble a functional endogenous acceptor and not from a defect in the synthesis of the sialyltransferase. Expand
Properties of membrane-associated sialyltransferase of Escherichia coli.
Kinetic studies as well as sugar nucleotide, metal ion, pH, ammonium sulfate, and thiol reagent requirements showed these two complexes contained functionally identical enzymatic activities, and lipididity of the lipid phase is apparently required for proper function of this membraneassociated enzyme complex. Expand
Influence of Growth Temperature on the Development of Escherichia coli Polysaccharide K Antigens
The established seventy-one Escherichia coli polysaccharide capsular K antigenic test strains were examined for the development of the K antigen after growth at 37 °C and 18 °C, and 43 antigens were developed at both temperatures. Expand
Protein measurement with the Folin phenol reagent.
Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein. Expand
Polysialic acid — a glycoprotein carbohydrate involved in neural adhesion and bacterial meningitis
Abstract Polysialic acid chains occur in glycoproteins of developing brain and at the surface of some bacteria causing meningitis. They may be involved in neural cell-adhesion and in the pathogenesisExpand
Modern biotechnology and health : perspectives for the year 2000
This modern biotechnology and health perspectives for the year 2000 can help you to solve the problem and can be one of the right sources to develop your writing skill. Expand