An in vivo model was used to investigate the regulation of tight junction (TJ) dynamics in the testis when adult rats were treated with CdCl(2). It was shown that the CdCl(2)-induced disruption of the blood-testis barrier (BTB) associated with a transient induction in testicular TGF-beta2 and TGF-beta3 (but not TGF-beta1) and the phosphorylated p38 mitogen… (More)
Fig. 3. Immunohistochemical localization of ZO-1 in the seminiferous epithelium of adult rat testes during CdCl2-induced BTB damage. (A) Low magnification of a cross section of a rat testis at 0 hours immunostained with an anti-ZO-1 antibody, and (B) a control section where normal rabbit serum was substituted for the primary antibody. (C-H) Micrographs at high magnifications of rat testes after CdCl2 treatment (3 mg/kg b.w.i.p.). Immunoreactive ZO-1 appears as reddishbrown precipitates localized at the basal compartment consistent with the site of the BTB (see arrowheads in A). These micrographs are typical of the results from at least 120 cross sections from three testes of different rats. Scale bar: (A,B) 200 µm; (C-H) 80 µm; (C inset, applies to insets in D-G) 20 µm.