Regulation of alpha2-macroglobulin expression in rat Sertoli cells and hepatocytes by germ cells in vitro.

Abstract

Germ cells isolated from rat testes by trypsinization have been shown to yield unwanted artifacts in biological assays, since conditioned media derived from these germ cells (germ cell-conditioned media [GCCM]) can modulate Sertoli cell secretory function because of the presence of residual trypsin. To determine whether germ cells themselves can modulate… (More)

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