Regulation of a cytochrome c2 isoform in wild-type and cytochrome c2 mutant strains of Rhodobacter sphaeroides.

@article{Rott1992RegulationOA,
  title={Regulation of a cytochrome c2 isoform in wild-type and cytochrome c2 mutant strains of Rhodobacter sphaeroides.},
  author={Marc A. Rott and John Fitch and Terry EuClaire Meyer and Timothy J. Donohue},
  journal={Archives of biochemistry and biophysics},
  year={1992},
  volume={292 2},
  pages={
          576-82
        }
}
In Rhodobacter sphaeroides, mutations that suppress the photosynthetic deficiency (spd mutations) of strains lacking cytochrome c2 (cyt c2) cause accumulation of a periplasmic cyt c2 isoform that has been designated isocytochrome c2 (isocyt c2). In this study, a new method for purification of both cyt c2 and isocyt c2 is described that uses periplasmic fluid as a starting material. In addition, antiserum to isocyt c2 has been used to demonstrate that all suppressor mutants contain an isocyt c2… CONTINUE READING
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