Regulation of Proteolytic Activity in Human Bone Marrow Stromal Cells by Basic Fibroblast Growth Factor, Interleukin-1, and Transforming Growth Factor f!

Abstract

Plasminogen activators (PAS) and/or plasmin may be involved in hematopoietic regulation. These enzymes release biologically relevant cytokines such as basic fibroblast growth factor (bFGF) from matrix and cell surfaces. In addition, transforming growth factor g (TGFg) and interleukin-lg (IL-1s) are converted from inactive to active forms by plasmin. Therefore, we studied the regulation of PAS and their specific inhibitors, PA inhibitor 1 (PAI-1) and PA inhibitor 2 (PAI-2). in human bone marrow stromal fibroblasts by IL-lS, bFGF, and TGFg. All three cytokines stimulated PA secretion. IL-1g at I@ U/mL increased urokinase (u-PA) levels approximately 10-fold, bFGF at 0.2 ng/mL also increased production IO-fold, but increased predominantly tissue PA (t-PA) expres-

6 Figures and Tables

Cite this paper

@inproceedings{Hannocks2003RegulationOP, title={Regulation of Proteolytic Activity in Human Bone Marrow Stromal Cells by Basic Fibroblast Growth Factor, Interleukin-1, and Transforming Growth Factor f!}, author={Melanie - Jane Hannocks and Lisa Oliver and Janice L . Gabrilove and E . Lynette}, year={2003} }