Regulation of Plant Acetyl-CoA Carboxylase by Adenylate Nucleotides.

  title={Regulation of Plant Acetyl-CoA Carboxylase by Adenylate Nucleotides.},
  author={K C Eastwell and Paul K. Stumpf},
  journal={Plant physiology},
  volume={72 1},
The assay of acetyl-CoA carboxylase (EC does not follow ideal zero-order kinetics when assayed in a crude extract from wheat (Triticum aestivum L.) germ. Our results show that the lack of ideality is the consequence of contamination by ATPase and adenylate kinase. These enzyme activities generate significant amounts of ADP and AMP in the assay mixture, thus limiting the availability of ATP for the carboxylase reaction. Moreover, ADP and AMP are competitive inhibitors, with respect to… CONTINUE READING


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