Regulation of N‐acetylaspartate and N‐acetylaspartylglutamate biosynthesis by protein kinase activators

@article{Arun2006RegulationON,
  title={Regulation of N‐acetylaspartate and N‐acetylaspartylglutamate biosynthesis by protein kinase activators},
  author={Peethambaran Arun and Chikkathur N. Madhavarao and John R. Moffett and M. A. Aryan Namboodiri},
  journal={Journal of Neurochemistry},
  year={2006},
  volume={98}
}
The neuronal dipeptide N‐acetylaspartylglutamate (NAAG) is thought to be synthesized enzymatically from N‐acetylaspartate (NAA) and glutamate. We used radiolabeled precursors to examine NAA and NAAG biosynthesis in SH‐SY5Y human neuroblastoma cells stimulated with activators of protein kinase A (dbcAMP; N6,2′‐O‐dibutyryl cAMP) and protein kinase C (PMA; phorbol‐12‐myristate‐13‐acetate). Differentiation over the course of several days with dbcAMP resulted in increased endogenous NAA levels and… 
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NAAG is present in a subpopulation of primary afferent spinal neurons and that its biosynthesis is mediated by a dipeptide synthetase, suggesting a precursor role for the large N‐acetylaspartate pool.
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TLDR
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TLDR
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