Enzyme Kinetics of Uridine Diphosphate Glucuronosyltransferases (UGTs).
- Biology, ChemistryMethods in molecular biology
Although extrapolation of the in vitro intrinsic clearance of a glucuronidated drug often underpredicts in vivo clearance, careful selection of in vitro experimental conditions and inclusion of extrahepatic glucuronidation may improve the predictivity of in vivo-in vitro extrapolation.
Microsomal UDP-glucuronyltransferase in rat liver: oxidative activation.
- Biology, Computer ScienceBasic & clinical pharmacology & toxicology
The results suggest that the main mechanism responsible for Fe(3+)/ascorbate-induced UDPGT activation is likely to be the promotion of protein sulfhydryl oxidation; this mechanism appears to be different from detergent-induced UDPGT activation.
Oxidation of barbiturates and the glucuronidation of 1-napthol in perfused rat liver and in microsomes
- Biology, Computer ScienceNaunyn-Schmiedeberg's Archives of Pharmacology
The rate of hexobarbital oxidation in perfused liver was slightly higher than in microsomes incubated with an NADPH regenerating system, indicating that the generation of reducing equivalents is not rate-limiting in livers of normal rats, and UDP-glucuronyltransferase is mostly latent within the cell.
Cellular Asymmetric Catalysis by UDP-glucuronosyltransferase 1A8 Shows Functional Localization to the Basolateral Plasma Membrane*
- Biology, ChemistryThe Journal of Biological Chemistry
The results suggest that some of the UGT1A8 enzyme is not residing in the endoplasmic reticulum but spans the plasma membrane, resulting in increased accessibility to compounds outside the cell.
ASCORBIC ACID DEFICIENCY AND HEPATIC UDP-
The effect of dietary ascorbate on hepatic UDP giucuronyitransferase (UDPGT) appears to be selective in that only certain isozymes of UDPGT are jeopardized. In this study, ascorbic acid deficiency…
Resolution of a Membrane-associated Thiosulphate-oxinig Complex of Thiobacillus novellus
- Biology, Chemistry
The thiosulphate-oxidizing system of Thiobncillus novellus was destroyed by treatment with proteolytic enzymes, phospholipase A, sodium deoxycholate and lysolecithin, indicating essential roles for…
UDP-glucuronosyltransferases (UGTs) in neuro-olfactory tissues: expression, regulation, and function
- BiologyDrug metabolism reviews
For the first time, using high-throughput in situ hybridization data generated by the Allen Brain Atlas, quantitative analysis of spatial distribution of UGT genes in the mouse brain is presented, including UGT isoforms not previously identified in the brain.
Vitamin A (retinol) status in the Gunn rat
- Biology, ChemistryArchives of Toxicology
The vitamin A reduction caused by TCDD was considerably less in the Gunn rat than in the Sprague-Dawley rat, and the results indicate that the Gunn rats is more resistant to TCDd than other strains of rat.
An enzymatic complex active in sulphite and thiosulphate oxidation by Rhodotorula sp.
- Biology, ChemistryArchives of Microbiology
An enzymatic complex active in thiosulphate and sulphite oxidation was isolated from the yeast Rhodotorula sp. and purified about 40-fold. The complex consists of two polypeptide chains of molecular…