Regulation of Gene Expression in Neurospora crassa with a Copper Responsive Promoter

@inproceedings{Lamb2013RegulationOG,
  title={Regulation of Gene Expression in Neurospora crassa with a Copper Responsive Promoter},
  author={Teresa M. Lamb and Justin Vickery and Deborah Bell-Pedersen},
  booktitle={G3},
  year={2013}
}
Precise control of gene expression is a powerful method to elucidate biological function, and protein overexpression is an important tool for industry and biochemistry. Expression of the Neurospora crassa tcu-1 gene (NCU00830), encoding a high-affinity copper transporter, is tightly controlled by copper availability. Excess copper represses, and copper depletion, via the use of a copper chelator, activates expression. The kinetics of induction and repression of tcu-1 are rapid, and the effects… CONTINUE READING
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