• Biology, Medicine
  • Published in
    The Journal of biological…
    1988

Regulation of Escherichia coli purF. Mutations that define the promoter, operator, and purine repressor gene.

@article{Rolfes1988RegulationOE,
  title={Regulation of Escherichia coli purF. Mutations that define the promoter, operator, and purine repressor gene.},
  author={Ronda J Rolfes and Howard Zalkin},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 36},
  pages={
          19649-52
        }
}
Mutations were constructed in vitro which identify the -35 promoter element and the operator site of the Escherichia coli purF operon as well as confirm the -10 promoter sequence. The operator was localized by a two-base change at positions -26 and -27, relative to the start of transcription. This mutation abolished repression of a purF-lacZ fusion. In the wild-type, repression of single copy and multicopy purF-lacZ constructs was equally effective. This indicates that cells contain a greater… CONTINUE READING

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