Regulation of Escherichia coli ornithine transcarbamylase by orotate.

  title={Regulation of Escherichia coli ornithine transcarbamylase by orotate.},
  author={David M. Knight and E. Elizabeth Jones},
  journal={The Journal of biological chemistry},
  volume={252 17},
Ornithine transcarbamylase from Escherichia coli, strain W, exhibits negative cooperativity with respect to ornithine, and the enzymatic activity is further regulated by orotate. The effect of orotate on ornithine transcarbamylase is dependent not only upon the carbamylphosphate concentration, but also upon the concentration of ornithine. At high concentrations of carbamylphosphate (10 mM), a conversion from negative cooperativity to positive cooperativity is observed with 10 mM orotate. At 1… CONTINUE READING