Regulation of Cloned Atp–Sensitive K Channels by Phosphorylation, Mgadp, and Phosphatidylinositol Bisphosphate (Pip2)

@article{Ribalet2000RegulationOC,
  title={Regulation of Cloned Atp–Sensitive K Channels by Phosphorylation, Mgadp, and Phosphatidylinositol Bisphosphate (Pip2)},
  author={Bernard Ribalet and Scott A. John and James N. Weiss},
  journal={The Journal of General Physiology},
  year={2000},
  volume={116},
  pages={391 - 410}
}
Kir6.2 channels linked to the green fluorescent protein (GFP) (Kir6. 2-GFP) have been expressed alone or with the sulfonylurea receptor SUR1 in HEK293 cells to study the regulation of K(ATP) channels by adenine nucleotides, phosphatidylinositol bisphosphate (PIP(2)), and phosphorylation. Upon excision of inside-out patches into a Ca(2+)- and MgATP-free solution, the activity of Kir6.2-GFP+SUR1 channels spontaneously ran down, first quickly within a minute, and then more slowly over tens of… CONTINUE READING

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Phosphoinositides Decrease Atp Sensitivity of the Cardiac Atp-Sensitive K+ Channel

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Competitive activation of Kir6.2ϩSUR1 channels by MgADP and PIP 2 Membrane phospholipid control of nucleotide sensitivity of K ATP channels

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