Regulation of Caco-2 cell proliferation by basolateral membrane epidermal growth factor receptors.

@article{Bishop1994RegulationOC,
  title={Regulation of Caco-2 cell proliferation by basolateral membrane epidermal growth factor receptors.},
  author={Warren P. Bishop and John T. Wen},
  journal={The American journal of physiology},
  year={1994},
  volume={267 5 Pt 1},
  pages={G892-900}
}
The epidermal growth factor (EGF) receptor is an important mediator of intestinal epithelial cell proliferation. We studied cell-surface localization of this molecule in Caco-2 cells and characterized cellular responses to apical or basolateral EGF stimulation. 125I-labeled EGF bound almost exclusively to a 180-kDa molecule, existing as a single high-affinity population by Scatchard analysis. On basolateral membranes 13- to 15-fold more ligand binding was seen. Apical/basolateral differences… CONTINUE READING