Regulation of Ca 2 Sparks by Ca 2 and Mg 2 in Mammalian and Amphibian Muscle . An RyR Isoform-specific Role in Excitation – Contraction Coupling ?

@inproceedings{Zhou2004RegulationOC,
  title={Regulation of Ca 2 Sparks by Ca 2 and Mg 2 in Mammalian and Amphibian Muscle . An RyR Isoform-specific Role in Excitation – Contraction Coupling ?},
  author={Jingsong Zhou and Bradley S Launikonis and Eduardo R{\'i}os and Gustavo Brum},
  year={2004}
}
Ca 2 and Mg 2 are important mediators and regulators of intracellular Ca 2 signaling in muscle. The effects of changes of cytosolic [Ca 2 ] or [Mg 2 ] on elementary Ca 2 release events were determined, as functions of concentration and time, in single fast-twitch permeabilized fibers of rat and frog. Ca 2 sparks were identified and their parameters measured in confocal images of fluo-4 fluorescence. Solutions with different [Ca 2 ] or [Mg 2 ] were rapidly exchanged while imaging. Faster and… CONTINUE READING