Regulation of CD163 on human macrophages: cross‐linking of CD163 induces signaling and activation

  title={Regulation of CD163 on human macrophages: cross‐linking of CD163 induces signaling and activation},
  author={Michel M. Heuvel and Cornelis P. Tensen and Jaco H. As and Timo K. Van Den Berg and Donna M. Fluitsma and Christine D. Dijkstra and Ed A. Döpp and Annette Droste and Floris A. Gaalen and Clemens Prof. Dr. Sorg and Petra H{\"o}gger and Robert H. J. Beelen},
  journal={Journal of Leukocyte Biology},
CD163 is a member of the group B scavenger receptor cysteine‐rich (SRCR) superfamily. This study describes aspects of the tissue distribution, the regulation of expression, and signal transduction after cross‐linking of this receptor at the cell surface of macrophages. CD163 showed an exclusive expression on resident macrophages (e.g., red pulp macrophages, alveolar macrophages). The expression was inducible on monocyte‐derived macrophages by glucocorticoids but not by interleukin‐4 (IL‐4… 
CD163-L1 Is an Endocytic Macrophage Protein Strongly Regulated by Mediators in the Inflammatory Response
CD163-L1 exhibits similarity to CD163 in terms of structure and regulated expression in cultured monocytes but shows clear differences compared with the known CD163 ligand preferences and expression pattern in the pool of tissue macrophages.
CD163: a regulated hemoglobin scavenger receptor with a role in the anti‐inflammatory response
Recent data indicate that solubleCD163 may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions and a role for soluble CD163 in immune suppression has been proposed.
Human monocytes express CD163, which is upregulated by IL-10 and identical to p155.
It is shown that >99% of all CD14 positive monocytes express CD163 and that monocyte derived dendritic cells express low levels of CD163, and that IL-10, like glucocorticoid-inducible member of the scavenger receptor cysteine-rich family of proteins, induces high CD163 expression on cultured human monocytes.
Tumor necrosis factor α‐converting enzyme (TACE/ADAM17) mediates ectodomain shedding of the scavenger receptor CD163
The present results provide a likely explanation for the strong empirical relationship between the sCD163 plasma level and infectious/inflammatory diseases relating to macrophage activity.
Interaction of CD163 with the regulatory subunit of casein kinase II (CKII) and dependence of CD163 signaling on CKII and protein kinase C
Inhibition studies using specific kinase inhibitors reveal that both CKII and PKC are involved in the CD163 signaling mechanism resulting in the secretion of proinflammatory cytokines.
Mycobacterium tuberculosis Induces CCL18 Expression in Human Macrophages
Up‐regulation of CCL18 and IL‐10 in macrophages by MTB may be involved in the recruitment of naïve T cells in association with local suppressive immunity against intracellular pathogens.
Soluble CD163 inhibits phorbol ester-induced lymphocyte proliferation.
A potential direct anti-inflammatory effect mediated by soluble CD163 is identified and statistically significantly inhibits phorbol ester-induced human T-lymphocyte activation, thus attenuating the immune response to the inflammatory mediator.
CD163 and its expanding functional repertoire.
The macrophage scavenger receptor CD163 functions as an innate immune sensor for bacteria.
These findings identify CD163 as a macrophage receptor for bacteria and suggest that, during bacterial infection, CD163 on resident tissue macrophages acts as an innate immune sensor and inducer of local inflammation.


Identification of a novel surface molecule, RM3/1, that contributes to the adhesion of glucocorticoid‐induced human monocytes to endothelial cells
The RM3/1‐molecule is a novel surface molecule that contributes to the adhesion of cortisone‐induced monocytes to LPS or cytokine‐stimulated endothelial cells.
Identification of a novel inducible cell-surface ligand of CD5 on activated lymphocytes
Immunoprecipitation, antibody, and recombinant protein blocking studies indicate that CD5L is distinct from CD72, which has been proposed to be a CD5 ligand, and propose a role for CD5 in the regulation of immune responses.
A monoclonal antibody to a novel differentiation antigen on human macrophages associated with the down-regulatory phase of the inflammatory process.
It is concluded that the antibody RM3/1 specifically detects a macrophage phenotype which seems to be associated with the healing phase of the inflammatory process.
Identification of the integral membrane protein RM3/1 on human monocytes as a glucocorticoid-inducible member of the scavenger receptor cysteine-rich family (CD163).
It is shown for the first time that CD163 is significantly up-regulated by glucocorticoids, suggesting that it might play an important role in inflammatory processes.
CSF‐1 signal transduction
  • J. Hamilton
  • Biology, Chemistry
    Journal of leukocyte biology
  • 1997
This review focuses mainly on recent studies of signal transduction events that are initiated on interaction of CSF‐1 and its receptor and the tyrosine autophosphorylation sites on c‐Fms identified to date.
Regulation of sialoadhesin expression on rat macrophages. Induction by glucocorticoids and enhancement by IFN-beta, IFN-gamma, IL-4, and lipopolysaccharide.
It is demonstrated that macrophages in the adrenal gland, the major site of endogenous GC production, express sialoadhesin, and that the response can be enhanced by IFN-beta, T cell-derived cytokines, or LPS.
Cell Activation Mediated by Glycosylphosphatidylinositol-Anchored or Transmembrane Forms of CD14
The results suggest that GPI anchoring and CD14 targeting to glycolipid-rich membrane microdomains are not required for LPS-mediated myeloid cell activation and may however be important for other signalling functions, such as those events reflected by antibody cross-linking.
A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily
The antibodies Ki‐M8, Ber‐Mac3, GHI/61 and SM4 define a human macrophage‐associated antigen with a relative molecular mass of 130000 which the authors designate M130, and the membrane protein encoded contains a leader peptide of 40 residues, a putative extracellular domain of 1003 residues, followed by a hydrophobic segment of 24 residues and a cytoplasmic domain of 49 residues.
Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products
The capacity of DCs to capture and process antigen could be modulated by exogenous stimuli was investigated and it was found that DCs respond to tumor necrosis factor alpha, CD40 ligand, IL-1, and lipopolysaccharide with a coordinate series of changes that include downregulation of macropinocytosis and Fc receptors, disappearance of the class II compartment, and upregulation of adhesion and costimulatory molecules.
Protein tyrosine kinase activation is required for lipopolysaccharide induction of cytokines in human blood monocytes.
It is suggested thatprotein kinase C and protein kinase A appear to have selective effects in the LPS induction of cytokines, whereas PTK is required for LPS induced of a broad spectrum of cytokine genes and NF-kappa B activation in monocytes.