Regulation of AP-2-synaptotagmin interaction by inositol high polyphosphates.

  title={Regulation of AP-2-synaptotagmin interaction by inositol high polyphosphates.},
  author={Akihiro Mizutani and Mitsunori Fukuda and Michio Niinobe and Katsuhiko Mikoshiba},
  journal={Biochemical and biophysical research communications},
  volume={240 1},
The inositol high-polyphosphate series (IHPS) inhibits neurotransmission through binding to the second C2 domain of synaptotagmins I and II(Syt), synaptic vesicle membrane proteins. We have revealed that several proteins, including alpha adaptins which are specific subunits of clathrin assembly protein, AP2, were eluted from mouse brain by affinity elution chromatography from the C2 domains of Syt II-immobilized Sepharose using 50 microM of InsP6. The interaction between Syt II and AP2 was more… CONTINUE READING