Regulation of 5'AMP-activated protein kinase activity and substrate utilization in exercising human skeletal muscle.

  title={Regulation of 5'AMP-activated protein kinase activity and substrate utilization in exercising human skeletal muscle.},
  author={J{\o}rgen F. P. Wojtaszewski and Christopher MacDonald and J Nielsen and Ylva Hellsten and David Grahame Hardie and Bruce E. Kemp and Bente Kiens and Erik A. Richter},
  journal={American journal of physiology. Endocrinology and metabolism},
  volume={284 4},
The metabolic role of 5'AMP-activated protein kinase (AMPK) in regulation of skeletal muscle metabolism in humans is unresolved. We measured isoform-specific AMPK activity and beta-acetyl-CoA carboxylase (ACCbeta) Ser(221) phosphorylation and substrate balance in skeletal muscle of eight athletes at rest, during cycling exercise for 1 h at 70% peak oxygen consumption, and 1 h into recovery. The experiment was performed twice, once in a glycogen-loaded (glycogen concentration approximately 900… 

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