Regulation by the autophosphorylation site in overexpressed pp60c-src.

@article{Kmiecik1988RegulationBT,
  title={Regulation by the autophosphorylation site in overexpressed pp60c-src.},
  author={Thomas E. Kmiecik and Patricia J. Johnson and David Shalloway},
  journal={Molecular and cellular biology},
  year={1988},
  volume={8 10},
  pages={4541-6}
}
We show that overexpressed pp60c-src is phosphorylated at Tyr-416 and has increased specific kinase activity when isolated from cells incubated with vanadate, a tyrosine phosphatase inhibitor. This supports the hypothesis that transient Tyr-416 phosphorylation modulates the activity of overexpressed pp60c-src in vivo. Mutagenesis indicates that Tyr-416 modulates pp60v-src activity as well.