Regulating gene expression using self-inactivating lentiviral vectors containing the mifepristone-inducible system.

@article{Sirin2003RegulatingGE,
  title={Regulating gene expression using self-inactivating lentiviral vectors containing the mifepristone-inducible system.},
  author={Olga Sirin and Frank Park},
  journal={Gene},
  year={2003},
  volume={323},
  pages={67-77}
}
Methods to regulate gene expression in vitro and in vivo are currently areas of intense research. The present study, therefore, was designed to determine the efficacy of transgene expression using the GeneSwitch mifepristone-regulatable system within the context of an integrating HIV-1 vector. Lentiviral transfer plasmids expressing the red (DsRed2) and green fluorescent protein (EGFP) markers were constructed for in vitro assessment on the basal and mifepristone-induced cell activation levels… CONTINUE READING