Regulated secretion: SNARE density, vesicle fusion and calcium dependence.

  title={Regulated secretion: SNARE density, vesicle fusion and calcium dependence.},
  author={Jens R Coorssen and Paul S. Blank and Fernando Albertorio and Ludmila Bezrukov and Irina Kolosova and Xiongfong Chen and Peter S. Backlund and Joshua Zimmerberg},
  journal={Journal of cell science},
  volume={116 Pt 10},
SNAREs such as VAMP, SNAP-25 and syntaxin are essential for intracellular trafficking, but what are their exact molecular roles and how are their interactions with other proteins manifest? Capitalizing on the differential sensitivity of SNAREs to exogenous proteases, we quantified the selective removal of identified SNAREs from native secretory vesicles without loss of fusion competence. Using previously established fusion assays and a high sensitivity immunoblotting protocol, we analyzed the… CONTINUE READING
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