Regulated overproduction of the GAL4 gene product greatly increases expression from galactose-inducible promoters on multi-copy expression vectors in yeast.

@article{Schultz1987RegulatedOO,
  title={Regulated overproduction of the GAL4 gene product greatly increases expression from galactose-inducible promoters on multi-copy expression vectors in yeast.},
  author={Loren D. Schultz and Kirsten Hofmann and Lawrence M. Mylin and Donna L. Montgomery and Ronald W. Ellis and James E. Hopper},
  journal={Gene},
  year={1987},
  volume={61 2},
  pages={123-33}
}
High-level, galactose-inducible expression originating from GAL promoters in Saccharomyces cerevisiae is mediated by highly specific interactions between the GAL4-coded protein and nucleotide sequences. The potential utility of recombinant GAL promoter/foreign gene constructions for the regulatable and high-level expression of foreign proteins in yeast is well recognized. However, the utility of this system is limited severely in the case of multiple copies of such constructions due to the very… CONTINUE READING

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