Regulated expression of heterologous genes inBacillus subtilis using the Tn10 encodedtet regulatory elements

@article{Geissendrfer1990RegulatedEO,
  title={Regulated expression of heterologous genes inBacillus subtilis using the Tn10 encodedtet regulatory elements},
  author={Manfred Geissend{\"o}rfer and Wolfgang Hillen},
  journal={Applied Microbiology and Biotechnology},
  year={1990},
  volume={33},
  pages={657-663}
}
SummaryTheEscherichia coli-derivedtet regulatory elements from Tn10 have been used to construct vectors allowing the regulated, inducible, high-level expression of foreign genes inBacillus subtilis. While the wild-typetet promoters are inactive inB. subtilis, a synthetic mutanttet sequence with improved promoter consensus sequences and upstream poly A blocks shows activity inB. subtilis. The expression of an indicatorcat gene is inducible by sublethal amounts of tetracycline, indicating that… CONTINUE READING
BETA

Figures, Tables, and Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 56 CITATIONS

Vectors for improved Tet repressor-dependent gradual gene induction or silencing in Staphylococcus aureus.

  • Microbiology
  • 2011
VIEW 6 EXCERPTS
CITES METHODS, BACKGROUND & RESULTS
HIGHLY INFLUENCED

The application of Tet repressor in prokaryotic gene regulation and expression

  • Microbial biotechnology
  • 2007
VIEW 6 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci.

  • Methods in molecular biology
  • 2014
VIEW 4 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

Regulon of the N-acetylglucosamine utilization regulator NagR in Bacillus subtilis.

  • Journal of bacteriology
  • 2011
VIEW 6 EXCERPTS
CITES METHODS & RESULTS
HIGHLY INFLUENCED

FILTER CITATIONS BY YEAR

1992
2019

CITATION STATISTICS

  • 10 Highly Influenced Citations

  • Averaged 2 Citations per year over the last 3 years

References

Publications referenced by this paper.
SHOWING 1-10 OF 30 REFERENCES

High level expression of Acinetobacter calcoaceticus mutarotase in Escherichia coli is achieved by improving the translational control sequence and removal of the signal sequence

R Schmucker, U Galland, M Will, W Hillen
  • Appl Microbiol Biotechnol
  • 1989
VIEW 1 EXCERPT

Expression of the Bacillus subtilis xyl operon is repressed at the level

D Girtner, M Geissend6rfer, W Hillen
  • 1988
VIEW 1 EXCERPT

Expression of the Bacillus subtilis xyl operon is repressed at the level of transcription and is induced by xylose

M rfer, W Hillen
  • J Bacteriol
  • 1988

Similar Papers

Loading similar papers…