Regulated expression of heterologous genes inBacillus subtilis using the Tn10 encodedtet regulatory elements

@article{Geissendrfer1990RegulatedEO,
  title={Regulated expression of heterologous genes inBacillus subtilis using the Tn10 encodedtet regulatory elements},
  author={Manfred Geissend{\"o}rfer and Wolfgang Hillen},
  journal={Applied Microbiology and Biotechnology},
  year={1990},
  volume={33},
  pages={657-663}
}
TheEscherichia coli-derivedtet regulatory elements from Tn10 have been used to construct vectors allowing the regulated, inducible, high-level expression of foreign genes inBacillus subtilis. While the wild-typetet promoters are inactive inB. subtilis, a synthetic mutanttet sequence with improved promoter consensus sequences and upstream poly A blocks shows activity inB. subtilis. The expression of an indicatorcat gene is inducible by sublethal amounts of tetracycline, indicating that the Tet… CONTINUE READING
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