Regulated expression of foreign genes in vaccinia virus under the control of bacteriophage T7 RNA polymerase and the Escherichia coli lac repressor.

@article{Alexander1992RegulatedEO,
  title={Regulated expression of foreign genes in vaccinia virus under the control of bacteriophage T7 RNA polymerase and the Escherichia coli lac repressor.},
  author={W. A. Alexander and Bernard Moss and Thomas R. Fuerst},
  journal={Journal of virology},
  year={1992},
  volume={66 5},
  pages={2934-42}
}
The gene encoding bacteriophage T7 RNA polymerase (T7gene1) was placed under the control of regulatory elements from the Escherichia coli lac operon to construct an inducible vaccinia virus expression system consisting entirely of prokaryotic transcriptional machinery. Regulated expression of T7 RNA polymerase was necessary to construct a stable recombinant vaccinia virus harboring a T7 promoter; otherwise, uncontrolled expression led to interference with endogenous virus replication. To this… CONTINUE READING

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Novel system for regulated high-level gene expression in vaccinia virus

W A Alexander, B Moss, T R Fuerst
Novel system for regulated high-level gene expression in vaccinia virus • 1991

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