Regulated expression and RNA processing of transcripts from the Srp20 splicing factor gene during the cell cycle

@article{Jumaa1997RegulatedEA,
  title={Regulated expression and RNA processing of transcripts from the Srp20 splicing factor gene during the cell cycle},
  author={Hassan Jumaa and Jean Louis Gu{\'e}net and Peter J. Nielsen},
  journal={Molecular and Cellular Biology},
  year={1997},
  volume={17},
  pages={3116 - 3124}
}
Eukaryotic splicing factors belonging to the SR family are essential splicing factors consisting of an N-terminal RNA-binding region and a C-terminal RS domain. They are believed to be involved in alternative splicing of numerous transcripts because their expression levels can influence splice site selection. We have characterized the structure and transcriptional regulation of the gene for the smallest member of the SR family, SRp20 (previously called X16). The mouse gene encoding SRp20… 
The splicing factor SRp20 modifies splicing of its own mRNA and ASF/SF2 antagonizes this regulation
TLDR
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It is demonstrated, for the first time, that an SR splicing factor, which represents a novel member of the SR family, is encoded by a functional retropseudogene.
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TLDR
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TLDR
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TLDR
This chapter will focus on the current mechanistic understanding of alternative splicing regulation by SR proteins and SR-related proteins and will discuss some of the questions that remain to be addressed in future research.
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TLDR
The results suggest that stress-inducible SRSF3-TR may participate in the acceleration of cell growth through facilitating c-Jun-mediated G1 progression under stressful conditions.
A genome landscape of SRSF 3-regulated splicing events and gene expression in human osteosarcoma U 2 OS cells
TLDR
The data indicate that SRSF3 affects a global change of gene expression to maintain cell homeostasis and significantly regulates the expression of at least 20 miRNAs, including a subset of oncogenic or tumor suppressive miRNAAs.
Alternative Splicing of Intron 3 of the Serine/Arginine-rich Protein 9G8 Gene
TLDR
These results, and the fact that the exon 3 and 4 ESE sequences are conserved in vertebrates, strongly suggest that the alternative splicing of intron 3 represents an important step in the regulation of the expression of 9G8.
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References

SHOWING 1-10 OF 65 REFERENCES
Identification and characterization of three members of the human SR family of pre‐mRNA splicing factors.
TLDR
Consistent with the postulated importance of SR proteins in alternative splicing in vivo, complex changes in the levels of mRNAs encoding the above SR proteins upon T cell activation are demonstrated, concomitant withChanges in the expression of alternatively spliced isoforms of CD44 and CD45.
Distinct functions of SR proteins in alternative pre-mRNA splicing.
TLDR
It is demonstrated that particular SR proteins have distinct functions in alternative pre-mRNA splicing in vitro, and this results suggest a fundamental role for SR proteins in the regulation of alternative splicing.
The structure and function of proteins involved in mammalian pre-mRNA splicing.
  • A. Krämer
  • Biology, Chemistry
    Annual review of biochemistry
  • 1996
TLDR
The structure and function of mammalian proteins are summarized which are essential components of the constitutive splicing machinery and which exert auxiliary functions in the recognition, selection, and juxtaposition of the splice sites and drive conformational changes during spliceosome assembly and catalysis.
The Gene Encoding Human Splicing Factor 9G8
TLDR
The results raise the possibility that alternative splicing of intron 3 provides a mechanism for modulation of the 9G8 function.
The Drosophila RNA-binding protein RBP1 is localized to transcriptionally active sites of chromosomes and shows a functional similarity to human splicing factor ASF/SF2.
TLDR
The general nuclear expression pattern, colocalization on chromosomes with RNA polymerase II, the similarity to ASF/SF2, SRp55, and B52, along with the effect on alternative splicing shown in vitro, suggest that rbp1 is involved in the processing of precursor mRNAs.
SR proteins: a conserved family of pre-mRNA splicing factors.
TLDR
It is demonstrated that four different proteins from calf thymus are able to restore splicing in the same splicing-deficient extract using several different pre-mRNA substrates, and a repeated protein sequence that encompasses an RNA recognition motif was observed.
Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family.
TLDR
It is shown that a recombinant 9G8 protein, expressed using a baculovirus vector and excluding other SR factors, rescues the splicing activity of a9G8‐depleted nuclear extract and an S100 cytoplasmic fraction, indicating that 9G 8 plays a crucial role in splicing, similar to that of the other SR splicing factors.
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