Regulated demethylation of the myoD distal enhancer during skeletal myogenesis.

@article{Brunk1996RegulatedDO,
  title={Regulated demethylation of the myoD distal enhancer during skeletal myogenesis.},
  author={Brian P. Brunk and David J Goldhamer and Charles P. Emerson},
  journal={Developmental biology},
  year={1996},
  volume={177 2},
  pages={490-503}
}
myoD is one of a family of four related basic helix-loop-helix transcription factors involved in the specification and differentiation of skeletal muscle. We previously identified a 258-bp distal enhancer that is sufficient for embryonic activation of myoD and is highly conserved between humans and mice. In this paper, we show using a modified bisulfite deamination/PCR amplification method that the distal myoD enhancer is completely unmethylated at all the CpG sites tested in myogenic cells and… CONTINUE READING