Regulated antisense RNA eliminates alpha-toxin virulence in Staphylococcus aureus infection.

@article{Ji1999RegulatedAR,
  title={Regulated antisense RNA eliminates alpha-toxin virulence in Staphylococcus aureus infection.},
  author={Yinduo Ji and Alberto Marra and Martin E. Rosenberg and Gary Woodnutt},
  journal={Journal of bacteriology},
  year={1999},
  volume={181 21},
  pages={
          6585-90
        }
}
The ability to selectively disrupt gene function remains a critical element in elucidating information regarding gene essentiality for bacterial growth and/or pathogenesis. In this study, we adapted a tet regulatory expression system for use in Staphylococcus aureus, with the goal of downregulating gene expression via induction of antisense RNA. We demonstrate that this system exhibits a 50- to 100-fold dose-dependent level of induction in bacterial cells grown in culture (i.e., in vitro) and… CONTINUE READING

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