Regular cellular distribution of plasmids by oscillating and filament-forming ParA ATPase of plasmid pB171.

@article{Ebersbach2006RegularCD,
  title={Regular cellular distribution of plasmids by oscillating and filament-forming ParA ATPase of plasmid pB171.},
  author={Gitte Ebersbach and Simon Ringgaard and Jakob M\oller-Jensen and Qing Wang and David J Sherratt and Kenn Gerdes},
  journal={Molecular microbiology},
  year={2006},
  volume={61 6},
  pages={1428-42}
}
Centromere-like loci from bacteria segregate plasmids to progeny cells before cell division. The ParA ATPase (a MinD homologue) of the par2 locus from plasmid pB171 forms oscillating helical structures over the nucleoid. Here we show that par2 distributes plasmid foci regularly along the length of the cell even in cells with many plasmids. In vitro, ParA binds ATP and ADP and has a cooperative ATPase activity. Moreover, ParA forms ATP-dependent filaments and cables, suggesting that ParA can… CONTINUE READING