Regiospecific transfer of tritium into 3H2O from labeled estrogens by mushroom tyrosinase.

@article{Jellinck1984RegiospecificTO,
  title={Regiospecific transfer of tritium into 3H2O from labeled estrogens by mushroom tyrosinase.},
  author={Peter H Jellinck and B I Norton and Jack Fishman},
  journal={Journal of steroid biochemistry},
  year={1984},
  volume={21 4},
  pages={361-5}
}
The specificity of mushroom tyrosinase in displacing 3H from estradiol and catechol estrogens labeled at C-1, C-2, C-4 or C-6,7 was investigated under various conditions. [2-3H]E2 Yielded significant amounts of 3H2O, in the presence of NADH, and the rate of 3H loss from the steroid paralleled that of the radioactivity remaining in the aqueous fraction after… CONTINUE READING