Regiospecific hydroxylation of isoflavones by cytochrome p450 81E enzymes from Medicago truncatula.

@article{Liu2003RegiospecificHO,
  title={Regiospecific hydroxylation of isoflavones by cytochrome p450 81E enzymes from Medicago truncatula.},
  author={Chang-Jun Liu and David V. Huhman and Lloyd W. Sumner and Richard A. Dixon},
  journal={The Plant journal : for cell and molecular biology},
  year={2003},
  volume={36 4},
  pages={471-84}
}
Mining of Medicago truncatula EST databases and screening of a root cDNA library led to the identification of three cytochrome p450 81E subfamily members. Two were functionally characterized by expression in yeast. The recombinant enzymes in yeast microsomes utilized the same isoflavone substrates, but produced different products hydroxylated at the 2' and/or 3' positions of the B-ring. When transiently expressed in alfalfa leaves, green fluorescent protein (GFP) fusions of the isoflavone 2… CONTINUE READING

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