Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system

@article{Girhard2009RegioselectiveBO,
  title={Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system},
  author={Marco Girhard and Kazuhiro Machida and Masashi Itoh and Rolf D. Schmid and Akira Arisawa and Vlada B Urlacher},
  journal={Microbial Cell Factories},
  year={2009},
  volume={8},
  pages={36 - 36}
}
BACKGROUND (+)-Nootkatone (4) is a high added-value compound found in grapefruit juice. Allylic oxidation of the sesquiterpene (+)-valencene (1) provides an attractive route to this sought-after flavoring. So far, chemical methods to produce (+)-nootkatone (4) from (+)-valencene (1) involve unsafe toxic compounds, whereas several biotechnological approaches applied yield large amounts of undesirable byproducts. In the present work 125 cytochrome P450 enzymes from bacteria were tested for… CONTINUE READING