• DOI:10.1113/expphysiol.2006.036228
• Corpus ID: 1971460

Regional sympathetic denervation affects the relation between canine local myocardial blood flow and oxygen consumption

@article{Alders2007RegionalSD,
  title={Regional sympathetic denervation affects the relation between canine local myocardial blood flow and oxygen consumption},
  author={David J. C. Alders and Richard N M Cornelussen and Frits W. Prinzen and Patricia A. C. Specht and Mark I M Noble and Angela J. Drake-Holland and Frans J. J. de Kanter and Johannes H G M van Beek},
  journal={Experimental Physiology},
  year={2007},
  volume={92}
}

• D. Alders, R. Cornelussen, +5 authors J. V. van Beek
• Published 1 May 2007
• Medicine, Biology
• Experimental Physiology

Myocardial blood flow and oxygen consumption are heterogeneously distributed. Perfusion and myocardial oxygen consumption are closely correlated in the normal heart. It is unknown how this metabolism–perfusion relation is influenced by sympathetic denervation. We investigated this question in seven chloralose‐anaesthetized dogs, 3–4 weeks after regional sympathetic denervation of the left circumflex coronary artery area of supply of the left ventricle. Measurements were made of local myocardial blood flow (MBF, in ml min−1 (g dry wt)−1), measured with microspheres, and myocardial oxygen consumption ( , in μmol min−1 (g dry wt)−1) in the same location, calculated from the 13C spectrum of tissue extracts after intracoronary infusion of 3–13C‐lactate. Since both innervated and denervated regions are subject to the same arterial pressure, lower blood flow indicates higher resistance. Mean MBF was 5.56 ml min−1 (g dry wt)−1 (heterogeneity of 3.47 ml min−1 (g dry wt)−1) innervated, 7.48 ml min−1 (g dry wt)−1 (heterogeneity of 3.62 ml min−1 (g dry wt)−1) denervated (n.s.). Significant linear relations were found between MBF and of individual samples within the innervated and denervated regions. The slopes of these relations were not significantly different, but the adjusted mean was significantly higher in the denervated regions (+1.92 ml min−1 (g dry wt)−1, an increase of 38% of the mean MBF at the pooled mean , P= 0.028, ANCOVA). The ratio (in ml μmol−1) was significantly higher, being 0.296 ± 0.167 ml μmol−1 in the denervated region compared with the innervated region, 0.216 ± 0.126 ml μmol−1, P= 0.0182, Mann–Whitney U test. These results indicate that sympathetic tone under chloralose anaesthesia imposes a moderate vasoconstrictive effect in the myocardium that is not detected by comparison of the mean blood flow or resistance.