Regional mutagenesis using Dissociation in maize.

  title={Regional mutagenesis using Dissociation in maize.},
  author={Kevin R Ahern and Prasit Deewatthanawong and Justin P Schares and Michael Muszynski and Rebecca Weeks and Erik Vollbrecht and Jon Duvick and Volker P Brendel and Thomas P. Brutnell},
  volume={49 3},
We describe genetic screens, molecular methods and web resources newly available to utilize Dissociation (Ds) as an insertional mutagen in maize. Over 1700 Ds elements have been distributed throughout the maize genome to serve as donor elements for local or regional mutagenesis. Two genetic screens are described to identify Ds insertions in genes-of-interest (goi). In scheme I, Ds is used to generate insertion alleles when a recessive reference allele is available. A Ds insertion will enable… CONTINUE READING

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