Regional localization on the human X of DNA segments cloned from flow sorted chromosomes.

@article{Kunkel1982RegionalLO,
  title={Regional localization on the human X of DNA segments cloned from flow sorted chromosomes.},
  author={Louis M. Kunkel and Umadevi Tantravahi and M E Eisenhard and Samuel A. Latt},
  journal={Nucleic acids research},
  year={1982},
  volume={10 5},
  pages={1557-78}
}
Fluorescence activated sorting of chromosomes from 49,XXXXY human lymphoblasts has been used to obtain DNA enriched for the human X. This DNA was cloned in lambda phage Charon 21A to obtain a library of approximately 60,000 pfu. Phage inserts free of human highly repeated DNA sequences are localized to different regions of the human X by two independent hybridization analyses. The first utilized comparative hybridization to rodent-human hybrid cell DNA samples containing all or known portions… CONTINUE READING