Regional effects on chimera formation in 454 pyrosequenced amplicons from a mock community

@article{Shin2014RegionalEO,
  title={Regional effects on chimera formation in 454 pyrosequenced amplicons from a mock community},
  author={Sunguk Shin and Tae Kwon Lee and Jung Min Han and Joonhong Park},
  journal={Journal of Microbiology},
  year={2014},
  volume={52},
  pages={566-573}
}
Chimeras are a frequent artifact in polymerase chain reaction and could be the underlying causes of erroneous taxonomic identifications, overestimated microbial diversity, and spurious sequences. However, little is known about the regional effects on chimera formation. Therefore, we investigated the chimera formation rates in different regions of phylogenetically important biomarker genes to test the regional effects on chimera formation. An empirical study of chimera formation rates was… CONTINUE READING
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