Regional differences in distribution of surface proteins over the bull spermatozoa.


Surface-radioiodinated bull spermatozoa were ultrasonicated and fractionated by Percoll-gradient centrifugation. The different fractions obtained were solubilized and analyzed by SDS-polyacrylamide gel electrophoresis. Three fractions containing sperm heads, midpieces, and membranes and small fragments of the principal pieces were obtained. The… (More)


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