Regional cell shape changes control form and function of Kupffer's vesicle in the zebrafish embryo.

@article{Wang2012RegionalCS,
  title={Regional cell shape changes control form and function of Kupffer's vesicle in the zebrafish embryo.},
  author={Guangliang Wang and M. Lisa Manning and Jeffrey D. Amack},
  journal={Developmental biology},
  year={2012},
  volume={370 1},
  pages={
          52-62
        }
}

Figures from this paper

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References

SHOWING 1-10 OF 61 REFERENCES
The Rho kinase Rock2b establishes anteroposterior asymmetry of the ciliated Kupffer's vesicle in zebrafish
TLDR
A Rho kinase gene, rock2b, is identified, which is required for normal AP patterning of KV and subsequent LR development in the embryo, and a link between AP patterned of the ciliated Kupffer's vesicle and LR patterner of the zebrafish embryo is suggested.
Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut
TLDR
The proposed KV is a transient embryonic `organ of asymmetry' that directs LR development by establishing a directional fluid flow and genetic analysis reveals novel roles for the T-box transcription factor no tail and the Nodal signaling pathway as upstream regulators of lrdr1 expression and KV morphogenesis.
Fluid dynamics in zebrafish Kupffer's vesicle
  • N. Okabe, Bo Xu, R. Burdine
  • Biology
    Developmental dynamics : an official publication of the American Association of Anatomists
  • 2008
TLDR
It is shown that cells lining both the dorsal roof and the ventral floor of Kupffer's vesicle possess posteriorly pointed cilia that rotate clockwise when viewed apically.
Origin and shaping of the laterality organ in zebrafish
TLDR
It is shown that Kupffer's vesicle (KV), the zebrafish organ of laterality, arises from a surface epithelium previously thought to adopt exclusively extra-embryonic fates, and a conserved progenitor role of the surface epithelialium during laterality organ formation in vertebrates is suggested.
Cilia-Driven Leftward Flow Determines Laterality in Xenopus
Analysis of Kupffer's vesicle in zebrafish embryos using a cave automated virtual environment
TLDR
The distribution of KV cilia in the Cave, an immersive virtual environment that displays stacks of confocal images in 3‐D, is analyzed and an asymmetrical distribution of cilia on the dorsal surface could explain the directionality of the flow, and could drive local differences in flow rate.
Planar Cell Polarity Enables Posterior Localization of Nodal Cilia and Left-Right Axis Determination during Mouse and Xenopus Embryogenesis
TLDR
Evidence is provided that vertebrate PCP proteins regulate planar polarity in the mouse ventral node and in the Xenopus gastrocoel roof plate, and a weakly penetrant Vangl1 mutant phenotype suggests that compromised VangL1 function may be associated with left-right laterality defects.
Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ
TLDR
It is shown that Wnt11- and Prickle1a-mediated planar cell polarity (PCP) signalling coordinates the formation of the zebrafish ciliated laterality organ by regulating adhesion properties between organ progenitor cells (the dorsal forerunner cells, DFCs).
Cilia-driven fluid flow in the zebrafish pronephros, brain and Kupffer's vesicle is required for normal organogenesis
TLDR
It is shown that cilia in the zebrafish larval kidney, the spinal cord and Kupffer's vesicle are motile, suggesting that fluid flow is a common feature of each of these organs and that the `nodal flow' mechanism of generating situs is conserved in non-mammalian vertebrates.
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