Regional assignment of the gene for diphtheria toxin sensitivity using subchromosomal fragments in microcell hybrids

  title={Regional assignment of the gene for diphtheria toxin sensitivity using subchromosomal fragments in microcell hybrids},
  author={H{\'e}l{\'e}ne C. Hayes and Yasufumi Kaneda and Tsuyoshi Uchida and Yoshio Okada},
Human x mouse microcell hybrids resistant to G418 were constructed between mouse hepatoma cells and human x mouse whole cell hybrids containing only intact human chromosome 5 and 22 with an integrated neor-gene. Among these, microcell hybrid BG15 produced four subclones, BG15-4, BG15-6, BG15-7 and BG15-9, which contained variously sized complements of human chromosome 5. BG15-6 contained an intact human chromosome 5, BG15-7 a deleted human chromosome 5 (5pter-q22) and BG15-4 and BG15-9 a… 

Monoclonal antibodies specific for human chromosome 5 obtained with a monochromosomal hybrid can be used to sort out cells containing the chromosome with a FACS

This method for sorting cells containing human chromosome 5 or an appropriate fragment of this chromosome from among human-rodent hybrid cells should be very useful in studies on gene expression, gene cloning and gene mapping.

Molecular analysis of the isochromosome 12P in the Pallister-Killian syndrome

An iso 12p chromosome from a patient with Pallister-Killian syndrome was successfully transferred into a mouse background by microcell-mediated chromosome transfer and this cell line should be a valuable tool for physical mapping of 12p-derived DNA fragments.

The Membrane Protein CD 9 / DRAP 27 Potentiates the Juxtacrine Growth Factor Activity of the Membrane-anchored Heparin-binding EGF-like Growth Factor

It is suggested that optimal expression of the juxtacrine growth activity of proHB-EGF/DTR requires co-expression of CD9/ DRAP27 and growth factor potentiation effects which have been observed previously for soluble growth factors also occurs at the level of cell surface associated growth factors.

The Anti-tumour Agent, Cisplatin, and its Clinically Ineffective Isomer, Transplatin, Produce Unique Gene Expression Profiles in Human Cells

Cisplatin treatment was shown to significantly up- or down-regulate a consistent subset of genes, and the identification of gene expression responses unique to clinically active compounds, like cisplatin, could thus greatly benefit the design and development of improved chemotherapeutics.



Selective transfer of individual human chromosomes to recipient cells

Two hypoxanthine phosphoribosyltransferase-deficient human cell lines were stably transfected with pSV2 gpt, a plasmid containing the selectable marker Escherichia coli xanthine-guanine phosphorosyl transferase (Eco gpt) to create a library of interspecies microcell hybrid clones, each clone containing a unique single human chromosome in a mouse background.

Integration of Ecogpt and SV40 early region sequences into human chromosome 17: a dominant selection system in whole cell and microcell human‐mouse hybrids.

The ability to construct human‐mouse somatic cell hybrids using a dominant selection system and transfer of single human chromosomes to mouse teratocarcinoma cells will allow examination of developmentally regulated human gene sequences after differentiation of such hybrids are demonstrated.

Transfer of Chinese hamster chromosome 1 to mouse cells and regional assignment of 7 genes: A combination of gene transfer and microcell fusion

We have used a combination of chromosome-mediated gene transfer and microcell fusion techniques to transfer Chinese hamster chromosome 1 to mouse cells. Microcell hybrids containing a single hamster

Integration of a dominant selectable marker into human chromosomes and transfer of marked chromosomes to mouse cells by microcell fusion

Monochromosomal hybrids, in which the human chromosome is maintained by selection, have been produced for chromosomes 2, 5, 16, and a rearranged chromosome involving a translocation between chromosomes 1 and 2.

Assignment of the human dihydrofolate reductase gene to the q11----q22 region of chromosome 5

Cells from a dihydrofolate reductase-deficient Chinese hamster ovary cell line were hybridized to human fetal skin fibroblast cells and it was demonstrated that the DHFR gene resides in the q11----q22 region.

Chromosomal assignment of the gene for human elongation factor 2.

It was confirmed by using two-dimensional gel electrophoresis that PA toxin resistance in hybrid cells was caused by the presence of EF-2 resistant to ADP-ribosylation by fragment A of diphtheria toxin, suggesting that the gene encodingEF-2 is located on human chromosome 19.

Assignment of human dihydrofolate reductase gene to band q23 of chromosome 5 and of related pseudogene ΨHD1 to chromosome 3

The chromosomal location of the human dihydrofolate reductase (DHFR; EC gene that is amplified in a methotrexate-resistant human cell line has been investigated by screening a number of

Genetic analysis of the cell surface: association of human chromosome 5 with sensitivity to diphtheria toxin in mouse-human somatic cell hybrids.

It is shown that the sensitivity of the hybrid cells is due to a gene or genes located on human chromosome 5, and mouse-human cell hybrids in which chromosome 5 is present are as sensitive to the toxin as human cells, which hybrids without chromosome 5 are as resistant as mouse cells.

Mapping genetic markers on human chromosome 19 using subchromosomal fragments in somatic cell hybrids.

A series of mouse-human somatic cell hybrid lines (WILF) derived from a hybrid that was originally thought to have chromosome 19 as its only human chromosome revealed that material from the X long arm is present in several cases.

Transfer of genetic information by purified metaphase chromosomes.

  • O. McbrideH. Ozer
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1973
Transfer of genetic information from isolated mammalian chromosomes to recipient cells has been demonstrated and colonies of cells containing hypoxanthine phosphoribosyl transferase appeared to represent progeny of individual cells that had ingested chromosomes, replicated, and expressed the hprt gene.