Regeneration of transgenic loblolly pine (Pinus taeda L.) from zygotic embryos transformed with Agrobacterium tumefaciens

@article{Tang2001RegenerationOT,
  title={Regeneration of transgenic loblolly pine (Pinus taeda L.) from zygotic embryos transformed with Agrobacterium tumefaciens},
  author={Wei Tang and Ronald R. Sederoff and Ross W. Whetten},
  journal={Planta},
  year={2001},
  volume={213},
  pages={981-989}
}
Embryos of 24 open-pollinated families of loblolly pine (Pinus teade L.) were used as explants to conduct in vitro regeneration. Then, Agrobacterium tumefaciens strain GV3101 harboring the plasmid pPCV6NFHygGUSINT was used to transform mature zygotic embryos of seven families of loblolly pine. The frequency of transformation varied among families infected with A. tumefaciens. The highest frequency (100%) of transient β-glucuronidase (GUS)-expressing embryos was obtained from family 11-1029 with… CONTINUE READING

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