Regeneration of plants from leaf explants of Cucumis melo cv. Pusa Sharbati

Abstract

Leaves of three different sizes excised from 14, 21, 28 and 35-day-old seedlings of Cucumis melo were cultured on a MS medium supplemented with a range and combination of growth regulators. Maximum shoot differentiation from the leaf explants occurred in the combined presence of BAP and 2iP at equimolar concentration of 1 μM. Regeneration potential of leaves declined with increasing size of the leaves and the age of the donor seedlings. For elongation the shoots were transferred to MS+BAP [1 μM]. Such shoots were rooted with 75% frequency on MS+IAA [0.5 μM]. The plants have been established in pots.

DOI: 10.1007/BF00269535

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Cite this paper

@article{Kathal1988RegenerationOP, title={Regeneration of plants from leaf explants of Cucumis melo cv. Pusa Sharbati}, author={Rekha Kathal and Satyendra Prasad Bhatnagar and Sant S. Bhojwani}, journal={Plant Cell Reports}, year={1988}, volume={7}, pages={449-451} }