Regeneration of hyaline cartilage by cell-mediated gene therapy using transforming growth factor beta 1-producing fibroblasts.

@article{Lee2001RegenerationOH,
  title={Regeneration of hyaline cartilage by cell-mediated gene therapy using transforming growth factor beta 1-producing fibroblasts.},
  author={K H Lee and S U Song and T S Hwang and Youngsuk Yi and In Suk Oh and J. Y. Lee and K. Choi and M. S. Choi and S. J. Kim},
  journal={Human gene therapy},
  year={2001},
  volume={12 14},
  pages={
          1805-13
        }
}
Transforming growth factor beta (TGF-beta) has been considered as a candidate for gene therapy of orthopedic diseases. The possible application of cell-mediated TGF-beta gene therapy as a new treatment regimen for degenerative arthritis was investigated. In this study, fibroblasts expressing active TGF-beta 1 were injected into the knee joints of rabbits with artificially made cartilage defects to evaluate the feasibility of this therapy for orthopedic diseases. Two to 3 weeks after the… 

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References

SHOWING 1-10 OF 28 REFERENCES
Articular cartilage repair by gene therapy using growth factor-producing mesenchymal cells.
TLDR
Stimulation of perichondrium-derived mesenchymal cells by transfer of growth factor cDNA in a partial-thickness defect model allows for satisfactory cartilage restoration by a repair tissue comparable with hyaline articular cartilage.
Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur
TLDR
Exogenous transforming growth factor-beta injections into the subperiosteal region of newborn rat femurs demonstrate that mesenchymal precursor cells in the periosteum are stimulated by TGF-beta to proliferate and differentiate, as occurs in embryologic bone formation and early fracture healing.
Stimulation of articular cartilage repair in established arthritis by local administration of transforming growth factor-beta into murine knee joints.
TLDR
This study demonstrates for the first time that local administration of TGF-beta into arthritic joints stimulates the replenishment of PGs in depleted cartilage.
Repair of Articular Cartilage Defects One Year After Treatment with Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2)*
TLDR
The results demonstrate that the addition of rhBMP-2 to the operative site after creation of a full-thickness defect results in an improvement in the histological appearance and composition of the extracellular matrix at one year postoperatively.
The Effect of Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2) on the Healing of Full-Thickness Defects of Articular Cartilage*
TLDR
The capacity of rhBMP-2 to accelerate the healing of full-thickness defects of articular cartilage and to improve the histological appearance and biochemical characteristics of the repair cartilage is described.
Use of recombinant human osteogenic protein-1 for the repair of subchondral defects in articular cartilage in goats.
TLDR
It is suggested that implantation of rhOP-1 promotes cartilage formation in subchondral defects in goats at 4 months after implantation, suggesting that rhop-1 could be a novel factor for regeneration of cartilage in articular cartilage defects.
Cell origin and differentiation in the repair of full-thickness defects of articular cartilage.
TLDR
The origin and differentiation of cells in the repair of three-millimeter-diameter, cylindrical, full-thickness drilled defects of articular cartilage were studied histologically in New Zealand White rabbits to demonstrate their origin from the primitive mesenchymal cells of the marrow.
Recombinant Transforming Growth Factor‐β1 Induces Endochondral Bone in the Baboon and Synergizes with Recombinant Osteogenic Protein‐1 (Bone Morphogenetic Protein‐7) to Initiate Rapid Bone Formation
TLDR
Evidence is provided for a novel function of TGF‐β1 in the primate and the scientific basis for synergistic molecular therapeutics for the rapid regeneration of cartilage and bone.
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