Regeneration of flowering plants from difficile lily protoplasts by means of a nurse culture

  title={Regeneration of flowering plants from difficile lily protoplasts by means of a nurse culture},
  author={Mitsugu Horita and Hitomi Morohashi and Fuminori Komai},
The regeneration of difficile lily protoplasts isolated from suspension cells of the Oriental hybrid lily (Lilium L.) cultivars Casablanca, Siberia and Acapulco was achieved by using the nurse-culture method. The divided protoplasts grew into colonies with nurse cells that have no regeneration ability, and developed to visible calli on a medium containing picloram. Many plantlets were formed on the calli after transfer of the proliferated calli to hormone-free medium. We were able to transplant… CONTINUE READING

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