Regeneration of fertile plants from protoplasts of different Arabidopsis thaliana genotypes

  title={Regeneration of fertile plants from protoplasts of different Arabidopsis thaliana genotypes},
  author={Brigitte Damm and Lothar Willmitzer},
  journal={Molecular and General Genetics MGG},
A protocol for obtaining regenerated fertile plants from mesophyll protoplasts of Arabidopsis thaliana is reported. Protoplasts were isolated from leaves of 21-to 28-day-old Arabidopsis plants grown in a controlled environment. Sustained divisions were achieved when protoplasts were embedded in beads formed by 1.4% sodium alginate in the presence of 50mM CaCl2 in 0.4 mannitol, which was then exchanged againts modified B5 medium. About 0.4%–0.6% of the protoplasts developed into colonies of… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 28 extracted citations


Publications referenced by this paper.
Showing 1-10 of 18 references

A revised medium for rapid growth and bioassays with tobacco tissue cultures

  • T Murashige, F Skoog
  • Physiol Plant
  • 1962
Highly Influential
3 Excerpts

Genetic resources in Arabidopsis

  • AR Kranz, B Kirchheim
  • Arabidopsis Inf Serv
  • 1987
1 Excerpt

Genetic resources in Arabidopsis . Arabidopsis Inf Serv 24 ( special issue ) Laibach F ( 1943 ) Zur Atiologie der Bliitenbildung

  • PJ Larkin
  • 1987

Some aspects of calcium-dependent regulation in plant metabolism

  • H Kauss
  • Annu Rev Plant Physiol
  • 1987

Similar Papers

Loading similar papers…