Regeneration of fertile plants from protoplasts of different Arabidopsis thaliana genotypes

@article{Damm2004RegenerationOF,
  title={Regeneration of fertile plants from protoplasts of different Arabidopsis thaliana genotypes},
  author={Brigitte Damm and Lothar Willmitzer},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={213},
  pages={15-20}
}
A protocol for obtaining regenerated fertile plants from mesophyll protoplasts of Arabidopsis thaliana is reported. Protoplasts were isolated from leaves of 21-to 28-day-old Arabidopsis plants grown in a controlled environment. Sustained divisions were achieved when protoplasts were embedded in beads formed by 1.4% sodium alginate in the presence of 50mM CaCl2 in 0.4 mannitol, which was then exchanged againts modified B5 medium. About 0.4%–0.6% of the protoplasts developed into colonies of… CONTINUE READING

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