Regeneration and transformation via Agrobacterium tumefaciens of the strawberry cultivar Chandler

@article{Barcel2004RegenerationAT,
  title={Regeneration and transformation via Agrobacterium tumefaciens of the strawberry cultivar Chandler},
  author={Marta Barcel{\'o} and Iman El-Mansouri and Jose Angel Mercado and Miguel Angel Quesada and Fernando Pliego Alfaro},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={54},
  pages={29-36}
}
The effects of growth regulator balance and culture conditions on the morphogenetic response of leaf disks from greenhouse grown plants of the strawberry cultivar Chandler, have been studied. Best results were obtained in the presence of 2.46 μM IBA and 8.88 μM BA, where 47% of the cultures regenerated after 16 weeks with 2.9 shoot colonies per regenerating leaf disk. Optimum incubation conditions included two weeks in the dark with subsequent transfer to light (40 μmol m-2 s-1, 16 h). The… CONTINUE READING