Refractive-index-mismatch induced aberrations in single-photon and two-photon microscopy and the use of aberration correction.

@article{Booth2001RefractiveindexmismatchIA,
  title={Refractive-index-mismatch induced aberrations in single-photon and two-photon microscopy and the use of aberration correction.},
  author={M J Booth and Theresa Wilson},
  journal={Journal of biomedical optics},
  year={2001},
  volume={6 3},
  pages={
          266-72
        }
}
We examine the effects of aberrations induced by a refractive index mismatch on the signal level and resolution of single-photon (1-p) and two-photon (2-p), conventional and confocal scanning microscopes. In particular, we consider the aberrations introduced by an interface between oil/glass and water. Resolution is defined in terms of enclosed fluorescence, rather than full-width half-maximum, revealing more useful information for heavily aberrated point spread functions (PSFs). It is shown… CONTINUE READING

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