Refolding proteins by gel filtration chromatography.

  title={Refolding proteins by gel filtration chromatography.},
  author={Milton H. Werner and G Marius Clore and Angela M. Gronenborn and Azusa Kondoh and Robert J. Fisher},
  journal={FEBS letters},
  volume={345 2-3},
We have developed a facile means for the refolding of milligram quantities of purified proteins that employs gel filtration chromatography. We demonstrate by electrophoretic mobility shift and NMR spectroscopy that human ETS-1 protein, bovine ribonuclease A and E. coli integration host factor can be refolded into the native conformation using this technique… CONTINUE READING